Histology/Pathology


The QTRN provides high quality histological services through its core facilities located within the academic setting.  These cores provide paraffin, frozen histological and immunohistological services, as well as computerized image processing and storage. The core also provides electron microscopic (EM) analysis and assists in experimental design and the development and interpretation of tests and their results. These services are available to the entire research community and industry.

An interpretive pathology service is also provided by special request (contact M. Chagnon).

 

Services include:

 

  • Processing and sectioning of paraffin-embedded specimens
  • Processing and sectioning of frozen tissues (in OCT)
  • Routine and Special stains
  • Immunohistochemical / immunofluorescence staining
  • Antibody titration/optimization
  • Consultation and Staining for immunohistochemistry
  • Digitization of data
  • Embedding for EM, thin sectioning and staining for colloidal gold stain
  • EM analysis by technician
  • Access to EM
  • Training investigators and staff
  • Consultation with Histology personnel

  Service request forms

Follow the link of the relevant service request form from the list below, fill out and either submit directly online or fill out and print the PDF file and FAX to 514-398-6769. You will receive an e-mail confirming reception of your request along with the turnaround time.


 

A consultation with the Histology personnel is available.

 

 

     Price list


 

      Service request forms (PDF)



 

General Information:

Paraffin-embedded tissue:

·          This technique hardens tissue and allows for very thin (4-5µm) sections.

·          Applicable for tissues fixed in most fixative

·          Tissue fixed using this method cannot be used to detect labile antigens, DNA/RNA isolation (Alternative: freezing of fresh tissue)


Frozen tissue

·        This technique hardens tissue to allow for very thin slicing.

·          Frozen unfixed tissue has excellent antigenicity but inferior morphology preservation.

·          Suitable for the detection of labile antigens and DNA/RNA can be isolated from tissues frozen in OCT

·          Not suitable for studies in which optimal tissue morphology is essential (alternative paraffin-embedding)

 

Useful Links


http://www.ihcworld.com/
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